Hotspot

petA-psbJ

LSC · 66678-67902 · 1225 bp

Back to Calophyllum

Pi 0.00514

MarkerSeek score 66.40

Primer available yes

Label petA-psbJ

Feature Metadata

label_name: petA-psbJ
feature_type: igs
region: LSC
length_bp: 1225
variable_sites: 9
pi: 0.00514
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psbJ_p1	GGCATCTGTTATTTTGGCACA	ATTCCGGATTGGGTTCATCC	42.9	50.0	57.4	57.6	1256-1336 bp	1.0	68.9
petA-psbJ_p2	TGGCATCTGTTATTTTGGCAC	ATTCCGGATTGGGTTCATCC	42.9	50.0	57.4	57.6	1257-1337 bp	1.0	68.9
petA-psbJ_p3	TGAGAAGGTTCAATTGTCCGA	ATTCCGGATTGGGTTCATCC	42.9	50.0	57.2	57.6	1205-1285 bp	1.0	67.6
petA-psbJ_p4	ACCCATTACGTATCCAAGGTC	ATTCCGGATTGGGTTCATCC	47.6	50.0	57.2	57.6	1290-1370 bp	1.0	67.0
petA-psbJ_p5	GACCCATTACGTATCCAAGGT	ATTCCGGATTGGGTTCATCC	47.6	50.0	57.2	57.6	1291-1371 bp	1.0	67.0