Hotspot

ycf4-cemA

LSC · 63789-64788 · 1000 bp

Back to Calophyllum

Pi 0.012

MarkerSeek score 56.80

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 1000
variable_sites: 18
pi: 0.012
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	GGGAGCCATTCCTTTGACTC	CCACCAGGGCAACAATACTA	55.0	50.0	58.2	57.2	1172-1247 bp	1.0	73.2
ycf4-cemA_p2	CCATTCCTTTGACTCGGACC	CCACCAGGGCAACAATACTA	55.0	50.0	58.3	57.2	1167-1242 bp	1.0	73.0
ycf4-cemA_p3	CCTTTGACTCGGACCAATGA	CCACCAGGGCAACAATACTA	50.0	50.0	57.5	57.2	1162-1237 bp	1.0	71.4
ycf4-cemA_p4	TCCTTTGACTCGGACCAATG	CCACCAGGGCAACAATACTA	50.0	50.0	57.5	57.2	1163-1238 bp	1.0	71.4
ycf4-cemA_p5	CATTCCTTTGACTCGGACCA	CCACCAGGGCAACAATACTA	50.0	50.0	57.5	57.2	1166-1241 bp	1.0	71.3