Hotspot

psaA-ycf3

LSC · 45572-46326 · 755 bp

Back to Calophyllum

Pi 0.00353

MarkerSeek score 61.50

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: LSC
length_bp: 755
variable_sites: 4
pi: 0.00353
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	GCCCATTCCTCGAAAGAAGT	CAGAGGCTTGGTTCGATCAA	50.0	50.0	57.9	57.9	948-954 bp	1.0	78.9
psaA-ycf3_p2	GCCCATTCCTCGAAAGAAGT	TGAAGATCACAAGGCGGTTC	50.0	50.0	57.9	58.2	861-867 bp	1.0	78.2
psaA-ycf3_p3	GCCCATTCCTCGAAAGAAGT	TTGAAGATCACAAGGCGGTT	50.0	45.0	57.9	57.7	862-868 bp	1.0	77.6
psaA-ycf3_p4	GCCCATTCCTCGAAAGAAGT	GCTATAGCGCTTACTCCTGG	50.0	55.0	57.9	57.6	907-913 bp	1.0	76.8
psaA-ycf3_p5	GCCCATTCCTCGAAAGAAGT	AGAGGCTTGGTTCGATCAAG	50.0	50.0	57.9	57.6	947-953 bp	1.0	76.5