Hotspot

psbZ-trnG-GCC

LSC · 39265-40148 · 884 bp

Back to Calophyllum

Pi 0.00932

MarkerSeek score 61.20

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 884
variable_sites: 12
pi: 0.00932
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 61.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	TCTCCTGATGGTTGGTCAAC	CGGATAGCGGGAATCGAAC	50.0	57.9	57.1	58.2	1033-1058 bp	1.0	66.6
psbZ-trnG-GCC_p2	CTCCTGATGGTTGGTCAACT	CGGATAGCGGGAATCGAAC	50.0	57.9	57.1	58.2	1032-1057 bp	1.0	66.5
psbZ-trnG-GCC_p3	TCTCCTGATGGTTGGTCAAC	GATAGCGGGAATCGAACCC	50.0	57.9	57.1	57.8	1031-1056 bp	1.0	66.4
psbZ-trnG-GCC_p4	TCTCCTGATGGTTGGTCAAC	GGATAGCGGGAATCGAACC	50.0	57.9	57.1	57.8	1032-1057 bp	1.0	66.4
psbZ-trnG-GCC_p5	CTCCTGATGGTTGGTCAACT	GATAGCGGGAATCGAACCC	50.0	57.9	57.1	57.8	1030-1055 bp	1.0	66.4