Hotspot

psbM-trnD-GUC

LSC · 32218-33279 · 1062 bp

Back to Calophyllum

Pi 0.00631

MarkerSeek score 60.90

Primer available yes

Label psbM-trnD-GUC

Feature Metadata

label_name: psbM-trnD-GUC
feature_type: igs
region: LSC
length_bp: 1062
variable_sites: 10
pi: 0.00631
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 60.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbM-trnD-GUC_p1	AGAATGAAGAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	45.0	50.0	55.2	58.5	1220-1227 bp	1.0	54.7
psbM-trnD-GUC_p2	ACTAGAATGAAGAGTGCAGTAGC	TTCAATTGGTCAGAGCACCG	43.5	50.0	58.0	58.5	1223-1230 bp	1.0	53.3
psbM-trnD-GUC_p3	AGGAACTAGAATGAAGAGTGCAG	TTCAATTGGTCAGAGCACCG	43.5	50.0	57.9	58.5	1227-1234 bp	1.0	52.6
psbM-trnD-GUC_p4	AGGAACTAGAATGAAGAGTGCA	TTCAATTGGTCAGAGCACCG	40.9	50.0	56.8	58.5	1227-1234 bp	1.0	52.0
psbM-trnD-GUC_p5	GGAACTAGAATGAAGAGTGCAGT	TTCAATTGGTCAGAGCACCG	43.5	50.0	58.2	58.5	1226-1233 bp	1.0	51.9