Hotspot

trnG-UCC-trnR-UCU

LSC · 10658-11333 · 676 bp

Back to Calophyllum

Pi 0.0134
MarkerSeek score 68.50
Primer available yes
Label trnG-UCC-trnR-UCU

Feature Metadata

label_name
trnG-UCC-trnR-UCU
feature_type
igs
region
LSC
length_bp
676
variable_sites
13
pi
0.0134
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.96
markerseek_score
68.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-UCC-trnR-UCU_p1 AGCCTTCCAAGCTAACGATG AGAAGACCTTTGTCCTATCCA 50.0 42.9 58.0 55.7 713-756 bp 1.0 58.7
trnG-UCC-trnR-UCU_p2 AGCCTTCCAAGCTAACGATG AGGTTTAGAAGACCTTTGTCCT 50.0 40.9 58.0 56.7 719-762 bp 1.0 58.4
trnG-UCC-trnR-UCU_p3 CCTAGCCTTCCAAGCTAACG AGAAGACCTTTGTCCTATCCA 55.0 42.9 58.1 55.7 716-759 bp 1.0 58.4
trnG-UCC-trnR-UCU_p4 CCTAGCCTTCCAAGCTAACG AGGTTTAGAAGACCTTTGTCCT 55.0 40.9 58.1 56.7 722-765 bp 1.0 58.2
trnG-UCC-trnR-UCU_p5 CCCTAGCCTTCCAAGCTAAC AGAAGACCTTTGTCCTATCCA 55.0 42.9 57.7 55.7 717-760 bp 1.0 56.2