Hotspot

trnS-UGA

Genome · 26786-26874 · 89 bp

Back to Calophaca

Pi 0.0449

MarkerSeek score 68.40

Primer available yes

Label trnS-UGA

Feature Metadata

label_name: trnS-UGA
feature_type: tRNA
region: Genome
length_bp: 89
variable_sites: 4
pi: 0.0449
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 68.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-UGA_p1	TGGTCATGTTCAATTCAGTGGA	ACCGAGCCAACCTAAATCAA	40.9	45.0	57.9	57.1	188 bp	1.0	58.0
trnS-UGA_p2	TGGTCATGTTCAATTCAGTGGA	AACCGAGCCAACCTAAATCA	40.9	45.0	57.9	57.1	189 bp	1.0	57.9
trnS-UGA_p3	TGGTCATGTTCAATTCAGTGGA	AAACCGAGCCAACCTAAATCA	40.9	42.9	57.9	57.9	190 bp	1.0	55.7
trnS-UGA_p4	TGGTCATGTTCAATTCAGTGGA	AACCGAGCCAACCTAAATCAA	40.9	42.9	57.9	57.9	189 bp	1.0	55.7
trnS-UGA_p5	TGGTCATGTTCAATTCAGTGG	ACCGAGCCAACCTAAATCAA	42.9	45.0	56.4	57.1	188 bp	1.0	54.2