Hotspot

rpl16

Genome · 82057-83642 · 1586 bp

Back to Calophaca

Pi 0.0123

MarkerSeek score 54.30

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: Genome
length_bp: 1586
variable_sites: 19
pi: 0.0123
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 54.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	GACAGCTCTGATCACTTCGG	GTCGAATGGCTCAGAGAAGG	55.0	55.0	58.1	58.1	2145-2174 bp	1.0	81.2
rpl16_p2	GCTCCGCTATTATCGGCTAC	GTCGAATGGCTCAGAGAAGG	55.0	55.0	58.1	58.1	2009-2038 bp	1.0	81.2
rpl16_p3	GCTCCGCTATTATCGGCTAC	TCGAGGGAATTGGACGGATA	55.0	50.0	58.1	57.9	2365-2394 bp	1.0	80.9
rpl16_p4	AACTGTCTCAATTCTCGGGC	GTCGAATGGCTCAGAGAAGG	50.0	55.0	57.9	58.1	2288-2317 bp	1.0	80.9
rpl16_p5	GACAGCTCTGATCACTTCGG	TCGAGGGAATTGGACGGATA	55.0	50.0	58.1	57.9	2501-2530 bp	1.0	80.8