Hotspot

trnL-UAG-rpl32

Genome · 125280-126028 · 749 bp

Back to Calophaca

Pi 0.0134

MarkerSeek score 66.60

Primer available yes

Label trnL-UAG-rpl32

Feature Metadata

label_name: trnL-UAG-rpl32
feature_type: igs
region: Genome
length_bp: 749
variable_sites: 10
pi: 0.0134
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-rpl32_p1	TCCTAAGAGCAGCGTGTCTA	TGGACAGCATTGAAAGCCTT	50.0	45.0	57.9	58.0	866-932 bp	1.0	80.0
trnL-UAG-rpl32_p2	TCCTAAGAGCAGCGTGTCTA	TTGGACAGCATTGAAAGCCT	50.0	45.0	57.9	58.0	867-933 bp	1.0	80.0
trnL-UAG-rpl32_p3	AGCAGCGTGTCTACCAATTT	TGGACAGCATTGAAAGCCTT	45.0	45.0	57.8	58.0	859-925 bp	1.0	79.5
trnL-UAG-rpl32_p4	AGCAGCGTGTCTACCAATTT	TTGGACAGCATTGAAAGCCT	45.0	45.0	57.8	58.0	860-926 bp	1.0	79.5
trnL-UAG-rpl32_p5	CTAAGAGCAGCGTGTCTACC	TGGACAGCATTGAAAGCCTT	55.0	45.0	57.8	58.0	864-930 bp	1.0	79.4