Hotspot

rrn16-trnA-UGC

Genome · 102403-104136 · 1734 bp

Back to Calophaca

Pi 0.0208
MarkerSeek score 56.50
Primer available yes
Label rrn16-trnA-UGC

Feature Metadata

label_name
rrn16-trnA-UGC
feature_type
igs
region
Genome
length_bp
1734
variable_sites
36
pi
0.0208
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
56.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rrn16-trnA-UGC_p1 CCGCGAATCCTCTTGAAAGA CCTCTACGTTCTTGACAGCC 50.0 55.0 58.0 58.0 2482-2489 bp 1.0 81.3
rrn16-trnA-UGC_p2 CCGCGAATCCTCTTGAAAGA CTCTACGTTCTTGACAGCCC 50.0 55.0 58.0 58.0 2481-2488 bp 1.0 81.3
rrn16-trnA-UGC_p3 AACTCCAAAAACCCGTCCTC CCTCTACGTTCTTGACAGCC 50.0 55.0 58.0 58.0 2201-2208 bp 1.0 81.3
rrn16-trnA-UGC_p4 AACTCCAAAAACCCGTCCTC CTCTACGTTCTTGACAGCCC 50.0 55.0 58.0 58.0 2200-2207 bp 1.0 81.3
rrn16-trnA-UGC_p5 CGTTGAGTTTGGAACCCTGA CTCTACGTTCTTGACAGCCC 50.0 55.0 58.0 58.0 2348-2355 bp 1.0 81.1