Hotspot

psbL-psbF

Genome · 63997-64018 · 22 bp

Back to Calophaca

Pi 0.0455

MarkerSeek score 78.60

Primer available yes

Label psbL-psbF

Feature Metadata

label_name: psbL-psbF
feature_type: igs
region: Genome
length_bp: 22
variable_sites: 1
pi: 0.0455
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 78.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbL-psbF_p1	TCATTTGGGTTTGATTGTGTCA	AGTACGATGGTTGGCTGTTC	36.4	50.0	56.7	57.9	133 bp	1.0	53.6
psbL-psbF_p2	TGTTCATTTGGGTTTGATTGTGT	AGTACGATGGTTGGCTGTTC	34.8	50.0	57.6	57.9	136 bp	1.0	53.0
psbL-psbF_p3	TCATTTGGGTTTGATTGTGTCA	CAGTACGATGGTTGGCTGTT	36.4	50.0	56.7	58.2	134 bp	1.0	52.8
psbL-psbF_p4	TCATTTGGGTTTGATTGTGTCA	GTACGATGGTTGGCTGTTCA	36.4	50.0	56.7	58.2	132 bp	1.0	52.8
psbL-psbF_p5	TCATTTGGGTTTGATTGTGTCA	TACGATGGTTGGCTGTTCAC	36.4	50.0	56.7	58.2	131 bp	1.0	52.8