Hotspot

atpH-atpF

Genome · 49818-50451 · 634 bp

Back to Calophaca

Pi 0.00324

MarkerSeek score 63.20

Primer available yes

Label atpH-atpF

Feature Metadata

label_name: atpH-atpF
feature_type: igs
region: Genome
length_bp: 634
variable_sites: 2
pi: 0.00324
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpH-atpF_p1	CCGGAAGCAGAGGACAAAAT	GATGGCCAGTAACTCAAGCA	50.0	50.0	58.2	57.9	790-801 bp	1.0	79.6
atpH-atpF_p2	CCGGAAGCAGAGGACAAAAT	CGGATGGCCAGTAACTCAAG	50.0	55.0	58.2	58.3	792-803 bp	1.0	77.8
atpH-atpF_p3	AGCAGAGGACAAAATACGGG	GATGGCCAGTAACTCAAGCA	50.0	50.0	57.6	57.9	785-796 bp	1.0	77.8
atpH-atpF_p4	TGGGCTGGTTGTAGCATTAG	GATGGCCAGTAACTCAAGCA	50.0	50.0	57.6	57.9	719-730 bp	1.0	77.8
atpH-atpF_p5	CCGGAAGCAGAGGACAAAAT	TGGCCAGTAACTCAAGCAAA	50.0	45.0	58.2	57.6	788-799 bp	1.0	77.7