Hotspot

trnE-UUC-trnY-GUA

Genome · 31813-31870 · 58 bp

Back to Calophaca

Pi 0.0172
MarkerSeek score 62.40
Primer available yes
Label trnE-UUC-trnY-GUA

Feature Metadata

label_name
trnE-UUC-trnY-GUA
feature_type
igs
region
Genome
length_bp
58
variable_sites
1
pi
0.0172
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
62.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnE-UUC-trnY-GUA_p1 AAGTATGCCCCCATCGTCTA TGGATCGCAAATTTTTGGGC 50.0 45.0 57.9 57.9 238 bp 1.0 78.8
trnE-UUC-trnY-GUA_p2 TCTCTTTCAAGGAGGCAACG TGGATCGCAAATTTTTGGGC 50.0 45.0 57.8 57.9 203 bp 1.0 78.3
trnE-UUC-trnY-GUA_p3 AAGTATGCCCCCATCGTCTA CCAGCGTAGACATATTGCCA 50.0 50.0 57.9 57.8 203 bp 1.0 77.7
trnE-UUC-trnY-GUA_p4 AAGTATGCCCCCATCGTCTA ACCAGCGTAGACATATTGCC 50.0 50.0 57.9 57.8 204 bp 1.0 77.7
trnE-UUC-trnY-GUA_p5 TCTCTTTCAAGGAGGCAACG CCAGCGTAGACATATTGCCA 50.0 50.0 57.8 57.8 168 bp 1.0 77.2