Hotspot

psbZ-trnS-UGA

Genome · 26428-26785 · 358 bp

Back to Calophaca

Pi 0
MarkerSeek score 42.90
Primer available yes
Label psbZ-trnS-UGA

Feature Metadata

label_name
psbZ-trnS-UGA
feature_type
igs
region
Genome
length_bp
358
variable_sites
0
pi
0
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
42.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnS-UGA_p1 CGACCAACCATCAGGAGAAG CAACCACTCAGCCATCTCTC 55.0 55.0 58.0 58.0 480-481 bp 1.0 79.9
psbZ-trnS-UGA_p2 CGACCAACCATCAGGAGAAG GAGCTATCAACCACTCAGCC 55.0 55.0 58.0 58.1 487-488 bp 1.0 79.6
psbZ-trnS-UGA_p3 CGACCAACCATCAGGAGAAG GGAGCTATCAACCACTCAGC 55.0 55.0 58.0 58.1 488-489 bp 1.0 79.6
psbZ-trnS-UGA_p4 CGACCAACCATCAGGAGAAG CAAGACCGGAGCTATCAACC 55.0 55.0 58.0 58.1 495-496 bp 1.0 79.6
psbZ-trnS-UGA_p5 CCAACCATCAGGAGAAGCAA CAACCACTCAGCCATCTCTC 50.0 55.0 57.8 58.0 477-478 bp 1.0 78.4