Hotspot

ycf63

Genome · 27500-28267 · 768 bp

Back to Caloglossa

Pi 0.23

MarkerSeek score 79.80

Primer available yes

Label ycf63

Feature Metadata

label_name: ycf63
feature_type: gene
region: Genome
length_bp: 768
variable_sites: 246
pi: 0.23
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf63_p1	AACCAGTGACCATCTGCTTG	ACCTGTTGATCCTCCTGACA	50.0	50.0	58.1	58.0	1102-1168 bp	1.0	91.0
ycf63_p2	AACGGGACTGATGAAGTTCG	ACCTGTTGATCCTCCTGACA	50.0	50.0	57.9	58.0	1463 bp	0.333333	52.5
ycf63_p3	CGGGACTGATGAAGTTCGTT	ACCTGTTGATCCTCCTGACA	50.0	50.0	57.9	58.0	1461 bp	0.333333	52.5
ycf63_p4	TGAGCTAACCGCCCCTAATA	ACCTGTTGATCCTCCTGACA	50.0	50.0	57.9	58.0	1128 bp	0.333333	52.4
ycf63_p5	CGGGACTGATGAAGTTCGTT	AAAAAGGACGCTCACCTGTT	50.0	45.0	57.9	57.9	1474 bp	0.333333	52.3