Hotspot

ycf45

Genome · 105636-107348 · 1713 bp

Back to Caloglossa

Pi 0.237

MarkerSeek score 79.00

Primer available yes

Label ycf45

Feature Metadata

label_name: ycf45
feature_type: gene
region: Genome
length_bp: 1713
variable_sites: 577
pi: 0.237
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 79.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf45_p1	CTGCGTGATGTAGAGTTGCT	GGTTGTTCATTTGGTGCAGG	50.0	50.0	58.0	57.8	2686 bp	0.333333	52.1
ycf45_p2	TCAGCACCAAGATCATCTGC	GGTTGTTCATTTGGTGCAGG	50.0	50.0	58.0	57.8	2585 bp	0.333333	51.8
ycf45_p3	AAAAGTTCCGTGGGTTCGAA	GGTTGTTCATTTGGTGCAGG	45.0	50.0	58.0	57.8	2773 bp	0.333333	51.8
ycf45_p4	GGAGAGGTGGTAGAGTTGGT	GGTTGTTCATTTGGTGCAGG	55.0	50.0	58.1	57.8	2825 bp	0.333333	51.6
ycf45_p5	AAGTTCCGTGGGTTCGAATC	GGTTGTTCATTTGGTGCAGG	50.0	50.0	58.2	57.8	2771 bp	0.333333	50.5