Hotspot

upp

Genome · 160702-161271 · 570 bp

Back to Caloglossa

Pi 0.281
MarkerSeek score 78.20
Primer available yes
Label upp

Feature Metadata

label_name
upp
feature_type
gene
region
Genome
length_bp
570
variable_sites
225
pi
0.281
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
upp_p1 TTGGTGACATATCCATGCCG AGCTCCTTGGGTAGAACCAT 50.0 50.0 58.0 58.0 1304 bp 0.333333 52.8
upp_p2 TTGGTGACATATCCATGCCG CAAGGACCAACCGGATTAGG 50.0 55.0 58.0 58.0 1396 bp 0.333333 52.8
upp_p3 TTGGTGACATATCCATGCCG AAGGACCAACCGGATTAGGA 50.0 50.0 58.0 58.0 1395 bp 0.333333 52.7
upp_p4 TTGGTGACATATCCATGCCG AGGACCAACCGGATTAGGAA 50.0 50.0 58.0 58.0 1394 bp 0.333333 52.7
upp_p5 TTGGTGACATATCCATGCCG ACAGCCTTGGCAAGAAAGAA 50.0 45.0 58.0 57.9 1238 bp 0.333333 52.5