Hotspot

tilS

Genome · 30289-31266 · 978 bp

Back to Caloglossa

Pi 0.256

MarkerSeek score 80.10

Primer available yes

Label tilS

Feature Metadata

label_name: tilS
feature_type: gene
region: Genome
length_bp: 978
variable_sites: 351
pi: 0.256
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
tilS_p1	GAGTGTTACCGCTGTTGTGA	TCAAGGCATAAGTCGTCTGC	50.0	50.0	58.1	58.0	2155 bp	0.333333	52.1
tilS_p2	GAGTGTTACCGCTGTTGTGA	CAAGGCATAAGTCGTCTGCT	50.0	50.0	58.1	58.0	2154 bp	0.333333	52.1
tilS_p3	GCGCTCCTTTATGTCCTTGT	TCAAGGCATAAGTCGTCTGC	50.0	50.0	58.3	58.0	2102 bp	0.333333	51.2
tilS_p4	GCGCTCCTTTATGTCCTTGT	CAAGGCATAAGTCGTCTGCT	50.0	50.0	58.3	58.0	2101 bp	0.333333	51.1
tilS_p5	TGGAATTGTCCTTGTGTCCC	TCAAGGCATAAGTCGTCTGC	50.0	50.0	57.7	58.0	2040 bp	0.333333	51.0