Hotspot

sufC

Genome · 68999-69766 · 768 bp

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Pi 0.226

MarkerSeek score 80.20

Primer available yes

Label sufC

Feature Metadata

label_name: sufC
feature_type: gene
region: Genome
length_bp: 768
variable_sites: 248
pi: 0.226
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 80.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
sufC_p1	ACAACTTCCAACAAGCGGAT	GCGGAATTGGAAGCCTTTTC	45.0	50.0	58.0	58.0	1612 bp	0.333333	53.2
sufC_p2	TGGACACAAGTGGAAACAGG	GCGGAATTGGAAGCCTTTTC	50.0	50.0	57.9	58.0	1715 bp	0.333333	52.9
sufC_p3	GGACACAAGTGGAAACAGGT	GCGGAATTGGAAGCCTTTTC	50.0	50.0	57.9	58.0	1714 bp	0.333333	52.9
sufC_p4	ACAACTTCCAACAAGCGGAT	AGAGTAGGTTCAGCTGCTCA	45.0	50.0	58.0	58.1	1687 bp	0.333333	52.6
sufC_p5	TTCCAACAAGCGGATACTGG	GCGGAATTGGAAGCCTTTTC	50.0	50.0	57.9	58.0	1607 bp	0.333333	52.4