Hotspot

rps2

Genome · 75051-75752 · 702 bp

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Pi 0.161

MarkerSeek score 81.50

Primer available yes

Label rps2

Feature Metadata

label_name: rps2
feature_type: gene
region: Genome
length_bp: 702
variable_sites: 157
pi: 0.161
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 81.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps2_p1	ACTGACGGGCAACAAAATCT	GGTCGTTTAATTCCTGCTGG	45.0	50.0	58.0	56.8	1282-1306 bp	0.666667	68.5
rps2_p2	GCTCCTGTTCTGATGCGTAA	GGTCGTTTAATTCCTGCTGG	50.0	50.0	58.0	56.8	1094 bp	0.333333	43.6
rps2_p3	CAGTCCATCATTCCAGCTCC	GGTCGTTTAATTCCTGCTGG	55.0	50.0	58.0	56.8	1109 bp	0.333333	43.4
rps2_p4	GTCCATCATTCCAGCTCCTG	GGTCGTTTAATTCCTGCTGG	55.0	50.0	58.0	56.8	1107 bp	0.333333	43.4
rps2_p5	CCTAGCTTAGCTCCAACGTG	GGTCGTTTAATTCCTGCTGG	55.0	50.0	58.1	56.8	1259 bp	0.333333	43.1