Hotspot

ntcA

Genome · 150572-151216 · 645 bp

Back to Caloglossa

Pi 0.276

MarkerSeek score 79.90

Primer available yes

Label ntcA

Feature Metadata

label_name: ntcA
feature_type: gene
region: Genome
length_bp: 645
variable_sites: 246
pi: 0.276
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ntcA_p1	GGATGTAAAGGCTTCTGCGA	GGGTTCGAGTCCCGTTATTC	50.0	55.0	58.0	58.1	1649 bp	0.333333	52.6
ntcA_p2	TTGCCGACCAACGATGTATT	GGGTTCGAGTCCCGTTATTC	45.0	55.0	57.9	58.1	1716 bp	0.333333	52.0
ntcA_p3	GGTCCCAAGGGAGTGAGTAT	GGGTTCGAGTCCCGTTATTC	55.0	55.0	58.1	58.1	1869 bp	0.333333	51.8
ntcA_p4	TCCCAAGGGAGTGAGTATCC	GGGTTCGAGTCCCGTTATTC	55.0	55.0	57.8	58.1	1867 bp	0.333333	51.4
ntcA_p5	CCCAAGGGAGTGAGTATCCT	GGGTTCGAGTCCCGTTATTC	55.0	55.0	57.8	58.1	1866 bp	0.333333	51.3