Hotspot

moeB

Genome · 146183-147262 · 1080 bp

Back to Caloglossa

Pi 0.283

MarkerSeek score 80.40

Primer available yes

Label moeB

Feature Metadata

label_name: moeB
feature_type: gene
region: Genome
length_bp: 1080
variable_sites: 425
pi: 0.283
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 80.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
moeB_p1	TTTTCTGTTGCCGTTTCAGC	AAACACGCCTTGAAGGACTT	45.0	45.0	57.8	57.9	1483 bp	0.333333	51.3
moeB_p2	TTTTCTGTTGCCGTTTCAGC	GAAGGACTTGAACCCTCGAC	45.0	55.0	57.8	57.9	1472 bp	0.333333	51.1
moeB_p3	TTTTCTGTTGCCGTTTCAGC	GGAAACCTACGTTCTGCCAA	45.0	50.0	57.8	58.1	1442 bp	0.333333	50.8
moeB_p4	TTTTCTGTTGCCGTTTCAGC	TTGGAAACCTACGTTCTGCC	45.0	50.0	57.8	58.1	1444 bp	0.333333	50.8
moeB_p5	TTTTCTGTTGCCGTTTCAGC	AACCTACGTTCTGCCAACTG	45.0	50.0	57.8	58.1	1439 bp	0.333333	50.7