Hotspot

infB

Genome · 85905-88076 · 2172 bp

Back to Caloglossa

Pi 0.266

MarkerSeek score 77.90

Primer available yes

Label infB

Feature Metadata

label_name: infB
feature_type: gene
region: Genome
length_bp: 2172
variable_sites: 807
pi: 0.266
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
infB_p1	AAGAGCGCGTATATTAGGGC	TCTCCTTCAGACTGTGCTGA	50.0	50.0	57.6	58.0	2559 bp	0.333333	50.4
infB_p2	AAGAGCGCGTATATTAGGGC	CTCCTTCAGACTGTGCTGAC	50.0	55.0	57.6	57.9	2558 bp	0.333333	49.8
infB_p3	AAGAGCGCGTATATTAGGGC	ACTGTGCTGACATTCCATCC	50.0	50.0	57.6	57.9	2549 bp	0.333333	49.5
infB_p4	AAGAGCGCGTATATTAGGGC	ACGCTTGAGGAAGTTGTGAG	50.0	50.0	57.6	58.1	2735 bp	0.333333	49.4
infB_p5	AAGAGCGCGTATATTAGGGC	CGCTTGAGGAAGTTGTGAGT	50.0	50.0	57.6	58.1	2734 bp	0.333333	49.4