Hotspot

accB

Genome · 107696-108190 · 495 bp

Back to Caloglossa

Pi 0.269

MarkerSeek score 77.40

Primer available yes

Label accB

Feature Metadata

label_name: accB
feature_type: gene
region: Genome
length_bp: 495
variable_sites: 187
pi: 0.269
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accB_p1	ATTGTACCAAAGACCGACCG	AATCATGAGCGTCTGCATGT	50.0	45.0	57.9	58.0	1541 bp	0.333333	52.3
accB_p2	CGACCGACCAACTCTACATG	AATCATGAGCGTCTGCATGT	55.0	45.0	58.1	58.0	1527 bp	0.333333	52.3
accB_p3	ATTGTACCAAAGACCGACCG	ACTGTCCAAAGTGAGCACTG	50.0	50.0	57.9	58.0	1607 bp	0.333333	52.3
accB_p4	CGACCGACCAACTCTACATG	ACTGTCCAAAGTGAGCACTG	55.0	50.0	58.1	58.0	1593 bp	0.333333	52.3
accB_p5	ATTGTACCAAAGACCGACCG	GTCCCACTATTGGCCAAACA	50.0	50.0	57.9	58.1	1736 bp	0.333333	52.0