Hotspot

psbY-rpl32

Genome · 15752-16004 · 253 bp

Back to Caloglossa

Pi 0.398

MarkerSeek score 72.20

Primer available yes

Label psbY-rpl32

Feature Metadata

label_name: psbY-rpl32
feature_type: igs
region: Genome
length_bp: 253
variable_sites: 111
pi: 0.398
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 72.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbY-rpl32_p1	TGCCCGCTTCTGTTAAAGTT	ACCCTGTGCTTAAAACATGA	45.0	40.0	58.0	55.2	1304 bp	0.333333	30.4
psbY-rpl32_p2	TTGCCCGCTTCTGTTAAAGT	ACCCTGTGCTTAAAACATGA	45.0	40.0	58.0	55.2	1305 bp	0.333333	30.4
psbY-rpl32_p3	AGTTCCCGTAGTTTGGCTTG	ACCCTGTGCTTAAAACATGA	50.0	40.0	58.1	55.2	1429 bp	0.333333	29.7
psbY-rpl32_p4	TGCCCGCTTCTGTTAAAGTT	AACCCTGTGCTTAAAACATGA	45.0	38.1	58.0	56.0	1305 bp	0.333333	28.9
psbY-rpl32_p5	TGCCCGCTTCTGTTAAAGTT	ACCCTGTGCTTAAAACATGAA	45.0	38.1	58.0	56.0	1304 bp	0.333333	28.9