Hotspot

ConsOrf4

Genome · 66647-67432 · 786 bp

Back to Caloglossa

Pi 0.355

MarkerSeek score 80.00

Primer available yes

Label ConsOrf4

Feature Metadata

label_name: ConsOrf4
feature_type: gene
region: Genome
length_bp: 786
variable_sites: 383
pi: 0.355
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 80.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ConsOrf4_p1	TGCGTATACCAATTTCGCCA	ACAGCATCTACGGCAACATT	45.0	45.0	58.0	57.9	1336-1360 bp	0.666667	76.0
ConsOrf4_p2	ACCAATTTCGCCATCTACCC	ACAGCATCTACGGCAACATT	50.0	45.0	57.9	57.9	1329-1353 bp	0.666667	75.8
ConsOrf4_p3	TGCGTATACCAATTTCGCCA	AGAGCCATCTGTAAATGCCG	45.0	50.0	58.0	58.0	1547 bp	0.333333	52.8
ConsOrf4_p4	ACCAATTTCGCCATCTACCC	AGAGCCATCTGTAAATGCCG	50.0	50.0	57.9	58.0	1540 bp	0.333333	52.6
ConsOrf4_p5	TGCGTATACCAATTTCGCCA	GGCAATGTGTATTGGGTGGA	45.0	50.0	58.0	58.2	1578 bp	0.333333	51.9