Hotspot

ConsOrf3

Genome · 159154-159639 · 486 bp

Back to Caloglossa

Pi 0.352

MarkerSeek score 79.90

Primer available yes

Label ConsOrf3

Feature Metadata

label_name: ConsOrf3
feature_type: gene
region: Genome
length_bp: 486
variable_sites: 222
pi: 0.352
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.92
markerseek_score: 79.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ConsOrf3_p1	TGTCGCGGAAGGTAATCAAG	AAGCGAGATGGTAAAGCGTT	50.0	45.0	58.0	57.9	1660 bp	0.333333	52.5
ConsOrf3_p2	GATTAAAGTGGGTGCCGCTA	AAGCGAGATGGTAAAGCGTT	50.0	45.0	58.0	57.9	1525 bp	0.333333	52.3
ConsOrf3_p3	TGTCGCGGAAGGTAATCAAG	TTTGGCCTAGAAACGATGCA	50.0	45.0	58.0	57.8	1373 bp	0.333333	51.8
ConsOrf3_p4	TGTCGCGGAAGGTAATCAAG	AGGCTTTATCTTTGCTGGCA	50.0	45.0	58.0	57.8	1204 bp	0.333333	51.5
ConsOrf3_p5	GATTAAAGTGGGTGCCGCTA	TTTGGCCTAGAAACGATGCA	50.0	45.0	58.0	57.8	1238 bp	0.333333	51.5