Hotspot

ycf1

SSC · 124859-130450 · 5592 bp

Back to Calochortus

Pi 0.0235

MarkerSeek score 53.40

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5592
variable_sites: 131
pi: 0.0235
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 53.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	AAATTAAAACGTGGCTCGCG	GGATAGCCCAGTTGACGAAG	45.0	55.0	58.0	58.1	768 bp	0.5	59.2
ycf1_p2	AAATTAAAACGTGGCTCGCG	CCGGGTGAATGGAAAGGAAA	45.0	50.0	58.0	58.1	828 bp	0.5	59.0
ycf1_p3	AAATTAAAACGTGGCTCGCG	AGCCCAGTTGACGAAGATTC	45.0	50.0	58.0	57.9	764 bp	0.5	58.9
ycf1_p4	AAATTAAAACGTGGCTCGCG	GCCCAGTTGACGAAGATTCT	45.0	50.0	58.0	57.9	763 bp	0.5	58.9
ycf1_p5	AAATTAAAACGTGGCTCGCG	AGAGATCCGGGTGAATGGAA	45.0	50.0	58.0	58.1	834 bp	0.5	58.7