Hotspot

cemA

LSC · 60782-61468 · 687 bp

Back to Calochortus

Pi 0.0277

MarkerSeek score 62.60

Primer available yes

Label cemA

Feature Metadata

label_name: cemA
feature_type: gene
region: LSC
length_bp: 687
variable_sites: 19
pi: 0.0277
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
cemA_p1	AGAATAAGGGTCCTTTTCAG	GGGAGATCCGATGAGTTCT	40.0	52.6	52.1	55.6	726 bp	1.0	42.1
cemA_p2	TAGAATAAGGGTCCTTTTCAG	GGGAGATCCGATGAGTTCT	38.1	52.6	52.2	55.6	727 bp	1.0	42.1
cemA_p3	AGAAATAGAATAAGGGTCCTTTTCAG	GGGAGATCCGATGAGTTCT	34.6	52.6	57.0	55.6	732 bp	1.0	42.1
cemA_p4	AGAAATAGAATAAGGGTCCTTTTCA	GGGAGATCCGATGAGTTCT	32.0	52.6	55.9	55.6	732 bp	1.0	42.1
cemA_p5	ATAGAATAAGGGTCCTTTTCAG	GGGAGATCCGATGAGTTCT	36.4	52.6	52.9	55.6	728 bp	1.0	42.1