Hotspot

ndhE-ndhG

SSC · 118980-119180 · 201 bp

Back to Calochortus

Pi 0.0471

MarkerSeek score 73.10

Primer available yes

Label ndhE-ndhG

Feature Metadata

label_name: ndhE-ndhG
feature_type: igs
region: SSC
length_bp: 201
variable_sites: 9
pi: 0.0471
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 73.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhE-ndhG_p1	AAGTACATGCTCGAACATCA	AGGTGCAATTACTATGGCTCG	40.0	47.6	54.8	58.2	240-250 bp	1.0	51.4
ndhE-ndhG_p2	AAGTACATGCTCGAACATCA	GGTGCAATTACTATGGCTCG	40.0	50.0	54.8	56.7	239-249 bp	1.0	50.0
ndhE-ndhG_p3	AAAGTACATGCTCGAACATCA	AGGTGCAATTACTATGGCTCG	38.1	47.6	55.6	58.2	241-251 bp	1.0	49.9
ndhE-ndhG_p4	AGTACATGCTCGAACATCAT	AGGTGCAATTACTATGGCTCG	40.0	47.6	54.6	58.2	239-249 bp	1.0	49.5
ndhE-ndhG_p5	AAGTACATGCTCGAACATCA	GGTGCAATTACTATGGCTCGT	40.0	47.6	54.8	58.4	239-249 bp	1.0	49.2