Hotspot

psaJ-rpl33

LSC · 67018-67436 · 419 bp

Back to Calochortus

Pi 0.0505

MarkerSeek score 74.30

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 419
variable_sites: 20
pi: 0.0505
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.95
markerseek_score: 74.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	AATCTCCCCTTACTGCCCAT	CGCCTACGAAAAGAGAGCTT	50.0	50.0	58.1	58.0	1543-1681 bp	1.0	83.4
psaJ-rpl33_p2	ACCCGTGACATTTTGTACCC	CGCCTACGAAAAGAGAGCTT	50.0	50.0	58.1	58.0	1489-1627 bp	1.0	83.4
psaJ-rpl33_p3	CCCGTGACATTTTGTACCCA	CGCCTACGAAAAGAGAGCTT	50.0	50.0	58.1	58.0	1488-1626 bp	1.0	83.4
psaJ-rpl33_p4	ACAGGATTTGAACCCGTGAC	CGCCTACGAAAAGAGAGCTT	50.0	50.0	58.1	58.0	1500-1638 bp	1.0	83.4
psaJ-rpl33_p5	CAGGATTTGAACCCGTGACA	CGCCTACGAAAAGAGAGCTT	50.0	50.0	58.1	58.0	1499-1637 bp	1.0	83.3