Hotspot

petA-psaJ

LSC · 62668-63505 · 838 bp

Back to Calochortus

Pi 0.0358

MarkerSeek score 71.00

Primer available yes

Label petA-psaJ

Feature Metadata

label_name: petA-psaJ
feature_type: igs
region: LSC
length_bp: 838
variable_sites: 30
pi: 0.0358
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 71.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petA-psaJ_p1	ATCCATTACGTGTCCAAGGC	GCTGGTATTCCTGTGATCGG	50.0	55.0	58.0	58.1	1032-1048 bp	1.0	81.0
petA-psaJ_p2	ATCCATTACGTGTCCAAGGC	ATTCCGGGTTAGGCTCATCT	50.0	50.0	58.0	58.2	983-999 bp	1.0	80.6
petA-psaJ_p3	CATTACGTGTCCAAGGCCTT	GCTGGTATTCCTGTGATCGG	50.0	55.0	58.2	58.1	1029-1045 bp	1.0	79.9
petA-psaJ_p4	CATTACGTGTCCAAGGCCTT	ATTCCGGGTTAGGCTCATCT	50.0	50.0	58.2	58.2	980-996 bp	1.0	79.5
petA-psaJ_p5	TTACGTGTCCAAGGCCTTTT	GCTGGTATTCCTGTGATCGG	45.0	55.0	57.6	58.1	1027-1043 bp	1.0	78.5