Hotspot

ndhC-trnV-UAC

LSC · 49419-50423 · 1005 bp

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Pi 0.0451
MarkerSeek score 77.40
Primer available yes
Label ndhC-trnV-UAC

Feature Metadata

label_name
ndhC-trnV-UAC
feature_type
igs
region
LSC
length_bp
1005
variable_sites
42
pi
0.0451
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.93
markerseek_score
77.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhC-trnV-UAC_p1 TGGTGCTAAAACTCCGGAAA CCGAGAAAGTCTACGGTTCG 45.0 55.0 57.4 58.1 1076-1140 bp 1.0 81.0
ndhC-trnV-UAC_p2 TTGGTGCTAAAACTCCGGAA CCGAGAAAGTCTACGGTTCG 45.0 55.0 57.4 58.1 1077-1141 bp 1.0 81.0
ndhC-trnV-UAC_p3 ATTGGTGCTAAAACTCCGGA CCGAGAAAGTCTACGGTTCG 45.0 55.0 57.1 58.1 1078-1142 bp 1.0 79.2
ndhC-trnV-UAC_p4 TTGGTGCTAAAACTCCGGAAA CCGAGAAAGTCTACGGTTCG 42.9 55.0 58.1 58.1 1077-1141 bp 1.0 77.6
ndhC-trnV-UAC_p5 TGGTGCTAAAACTCCGGAAAT CCGAGAAAGTCTACGGTTCG 42.9 55.0 57.9 58.1 1076-1140 bp 1.0 77.0