Hotspot

trnS-GCU-trnG-GCC

LSC · 7660-8895 · 1236 bp

Back to Calochortus

Pi 0.186
MarkerSeek score 88.70
Primer available yes
Label trnS-GCU-trnG-GCC

Feature Metadata

label_name
trnS-GCU-trnG-GCC
feature_type
igs
region
LSC
length_bp
1236
variable_sites
201
pi
0.186
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.87
markerseek_score
88.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-GCC_p1 TAGCGGAGAGAGAGGGATTC TGAAAAACCGAAAGACCCCT 55.0 45.0 57.7 57.3 1387-1406 bp 1.0 83.8
trnS-GCU-trnG-GCC_p2 CGGAGAGAGAGGGATTCGAA TGAAAAACCGAAAGACCCCT 55.0 45.0 58.3 57.3 1384-1403 bp 1.0 83.5
trnS-GCU-trnG-GCC_p3 GATAGCGGAGAGAGAGGGAT TGAAAAACCGAAAGACCCCT 55.0 45.0 57.5 57.3 1389-1408 bp 1.0 82.0
trnS-GCU-trnG-GCC_p4 TAGCGGAGAGAGAGGGATTC TGAAAAACCGAAAGACCCCTT 55.0 42.9 57.7 58.0 1387-1406 bp 1.0 81.6
trnS-GCU-trnG-GCC_p5 CGGAGAGAGAGGGATTCGAA TGAAAAACCGAAAGACCCCTT 55.0 42.9 58.3 58.0 1384-1403 bp 1.0 81.3