Hotspot

psbI-trnS-GCU

LSC · 7453-7569 · 117 bp

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Pi 0.094
MarkerSeek score 84.70
Primer available yes
Label psbI-trnS-GCU

Feature Metadata

label_name
psbI-trnS-GCU
feature_type
igs
region
LSC
length_bp
117
variable_sites
11
pi
0.094
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
84.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbI-trnS-GCU_p1 GCAATCCTGGACGTGAAGAA GGGTTCGAATCCCTCTCTCT 50.0 55.0 58.2 58.2 163-180 bp 1.0 81.9
psbI-trnS-GCU_p2 GCAATCCTGGACGTGAAGAA TTCGAATCCCTCTCTCTCCG 50.0 55.0 58.2 58.3 160-177 bp 1.0 81.1
psbI-trnS-GCU_p3 GTTTGGCAAGCTGCTGTAAG GGGTTCGAATCCCTCTCTCT 50.0 55.0 57.9 58.2 427-448 bp 1.0 80.7
psbI-trnS-GCU_p4 GGGGTTGGGAGAGTTCAAAG GGGTTCGAATCCCTCTCTCT 55.0 55.0 58.1 58.2 707-728 bp 1.0 79.8
psbI-trnS-GCU_p5 GTTTGGCAAGCTGCTGTAAG TTCCCGGCCATTACTTAAGC 50.0 50.0 57.9 58.0 1448 bp 0.5 59.2