Hotspot

ycf1

Genome · 104754-111659 · 6906 bp

Back to Calocedrus

Pi 0.0129

MarkerSeek score 45.60

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: Genome
length_bp: 6906
variable_sites: 89
pi: 0.0129
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 45.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GCTATGGCTGGTTGCTTTAG	GACCTTTAAGTCGTTGGGAGA	50.0	47.6	56.9	57.3	204 bp	1.0	62.6
ycf1_p2	GCTATGGCTGGTTGCTTTAG	AGACCTTTAAGTCGTTGGGAG	50.0	47.6	56.9	57.3	205 bp	1.0	62.5
ycf1_p3	GCTATGGCTGGTTGCTTTAG	ACCTTTAAGTCGTTGGGAGA	50.0	45.0	56.9	56.1	203 bp	1.0	60.7
ycf1_p4	GCTATGGCTGGTTGCTTTAG	AGACCTTTAAGTCGTTGGGA	50.0	45.0	56.9	56.1	205 bp	1.0	60.7
ycf1_p5	GCTATGGCTGGTTGCTTTAG	GACCTTTAAGTCGTTGGGAGAA	50.0	45.5	56.9	58.0	204 bp	1.0	59.9