Hotspot

trnL-CAA-accD

Genome · 112403-113770 · 1368 bp

Back to Calocedrus

Pi 0.112

MarkerSeek score 80.80

Primer available yes

Label trnL-CAA-accD

Feature Metadata

label_name: trnL-CAA-accD
feature_type: igs
region: Genome
length_bp: 1368
variable_sites: 130
pi: 0.112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.85
markerseek_score: 80.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-CAA-accD_p1	CACGCGACACTCAAAATGTC	TTCTCGTATGTGCTTCTGGC	50.0	50.0	58.1	58.0	1681-1807 bp	1.0	89.7
trnL-CAA-accD_p2	GACACGCGACACTCAAAATG	TTCTCGTATGTGCTTCTGGC	50.0	50.0	58.1	58.0	1683-1809 bp	1.0	89.6
trnL-CAA-accD_p3	CACGCGACACTCAAAATGTC	AGTATGGGATCGGTAGTGGG	50.0	55.0	58.1	58.0	1754-1880 bp	1.0	89.4
trnL-CAA-accD_p4	CACGCGACACTCAAAATGTC	GACGCTGGTGTCGAACTAAT	50.0	50.0	58.1	58.0	1955-2081 bp	1.0	88.9
trnL-CAA-accD_p5	GACACGCGACACTCAAAATG	GACGCTGGTGTCGAACTAAT	50.0	50.0	58.1	58.0	1957-2083 bp	1.0	88.9