Hotspot

psaJ-rpl33

Genome · 98900-99205 · 306 bp

Back to Calocedrus

Pi 0.044

MarkerSeek score 78.00

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: Genome
length_bp: 306
variable_sites: 11
pi: 0.044
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.82
markerseek_score: 78.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTTTTCCCAGATGCCCTGAC	ACATTTCCACTCTTGGCCAT	50.0	45.0	58.1	57.4	309-362 bp	1.0	81.1
psaJ-rpl33_p2	ATATCTTTCCACAGCGCCTG	ACATTTCCACTCTTGGCCAT	50.0	45.0	58.0	57.4	385-438 bp	1.0	80.9
psaJ-rpl33_p3	CATATCTTTCCACAGCGCCT	ACATTTCCACTCTTGGCCAT	50.0	45.0	58.0	57.4	386-439 bp	1.0	80.9
psaJ-rpl33_p4	GATGCCCTGACTTTTCCCTT	ACATTTCCACTCTTGGCCAT	50.0	45.0	57.8	57.4	300-353 bp	1.0	80.0
psaJ-rpl33_p5	ACATATCTTTCCACAGCGCC	ACATTTCCACTCTTGGCCAT	50.0	45.0	58.3	57.4	387-440 bp	1.0	78.6