Hotspot

psbJ-psbL

Genome · 96614-96792 · 179 bp

Back to Calocedrus

Pi 0.094

MarkerSeek score 66.40

Primer available yes

Label psbJ-psbL

Feature Metadata

label_name: psbJ-psbL
feature_type: igs
region: Genome
length_bp: 179
variable_sites: 11
pi: 0.094
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.65
markerseek_score: 66.40

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbJ-psbL_p1	AGGGATTCTTCCGGTGGTAT	TAGCCTCTATTGGGGGTTGT	50.0	50.0	57.8	57.7	214-276 bp	1.0	83.8
psbJ-psbL_p2	AGGGATTCTTCCGGTGGTAT	ACTAGCCTCTATTGGGGGTT	50.0	50.0	57.8	57.4	216-278 bp	1.0	81.3
psbJ-psbL_p3	GAGGGATTCTTCCGGTGGTA	TAGCCTCTATTGGGGGTTGT	55.0	50.0	58.5	57.7	215-277 bp	1.0	81.3
psbJ-psbL_p4	AGGGATTCTTCCGGTGGTAT	AGCCTCTATTGGGGGTTGTT	50.0	50.0	57.8	58.6	213-275 bp	1.0	81.1
psbJ-psbL_p5	GAGGGATTCTTCCGGTGGTA	ACTAGCCTCTATTGGGGGTT	55.0	50.0	58.5	57.4	217-279 bp	1.0	78.8