Hotspot

rpl36-rps11

Genome · 85129-85287 · 159 bp

Back to Calocedrus

Pi 0.0503

MarkerSeek score 81.30

Primer available yes

Label rpl36-rps11

Feature Metadata

label_name: rpl36-rps11
feature_type: igs
region: Genome
length_bp: 159
variable_sites: 8
pi: 0.0503
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 81.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps11_p1	AAGTGCCGCCTAATTCGTAG	CTTGTAGCTCTACGTGGCAC	50.0	55.0	58.1	58.4	257-259 bp	1.0	79.0
rpl36-rps11_p2	AAGTGCCGCCTAATTCGTAG	TCTTGTAGCTCTACGTGGCA	50.0	50.0	58.1	58.5	258-260 bp	1.0	78.3
rpl36-rps11_p3	CGAAAAGTGCCGCCTAATTC	CTTGTAGCTCTACGTGGCAC	50.0	55.0	57.8	58.4	261-263 bp	1.0	78.1
rpl36-rps11_p4	GAAAAGTGCCGCCTAATTCG	CTTGTAGCTCTACGTGGCAC	50.0	55.0	57.8	58.4	260-262 bp	1.0	78.1
rpl36-rps11_p5	AATACGTGCTTCTGTTCGCA	CTTGTAGCTCTACGTGGCAC	45.0	55.0	58.2	58.4	288-290 bp	1.0	77.6