Hotspot

ycf2-rpl23

Genome · 77987-78623 · 637 bp

Back to Calocedrus

Pi 0.18

MarkerSeek score 86.80

Primer available yes

Label ycf2-rpl23

Feature Metadata

label_name: ycf2-rpl23
feature_type: igs
region: Genome
length_bp: 637
variable_sites: 107
pi: 0.18
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 86.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf2-rpl23_p1	TGATTGTTTATTGGCGGAAGG	TCTTTGTTTTATTCGATCCCGA	42.9	36.4	56.9	55.8	792-833 bp	1.0	52.0
ycf2-rpl23_p2	TGATTGTTTATTGGCGGAAGGA	TCTTTGTTTTATTCGATCCCGA	40.9	36.4	58.3	55.8	792-833 bp	1.0	52.0
ycf2-rpl23_p3	TGATTGTTTATTGGCGGAAGG	TGATCTTTGTTTTATTCGATCCCG	42.9	37.5	56.9	57.6	795-836 bp	1.0	52.0
ycf2-rpl23_p4	GATTGTTTATTGGCGGAAGGA	TCTTTGTTTTATTCGATCCCGA	42.9	36.4	56.6	55.8	791-832 bp	1.0	52.0
ycf2-rpl23_p5	TTGATTGTTTATTGGCGGAAGG	TCTTTGTTTTATTCGATCCCGA	40.9	36.4	57.6	55.8	793-834 bp	1.0	52.0