Hotspot

rrn16-trnV-GAC

Genome · 64726-65556 · 831 bp

Back to Calocedrus

Pi 0.0531

MarkerSeek score 69.90

Primer available yes

Label rrn16-trnV-GAC

Feature Metadata

label_name: rrn16-trnV-GAC
feature_type: igs
region: Genome
length_bp: 831
variable_sites: 32
pi: 0.0531
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.73
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rrn16-trnV-GAC_p1	GGGCAGGTTCTTACGCATTA	ACCTTGACGTGGTGAAAGTC	50.0	50.0	58.0	58.1	997-1055 bp	1.0	85.9
rrn16-trnV-GAC_p2	CCTACAGACACTTTACGCCC	ACCTTGACGTGGTGAAAGTC	55.0	50.0	58.0	58.1	1433-1491 bp	1.0	85.5
rrn16-trnV-GAC_p3	CCTACAGACACTTTACGCCC	CCTTGACGTGGTGAAAGTCA	55.0	50.0	58.0	58.1	1432-1490 bp	1.0	85.5
rrn16-trnV-GAC_p4	GTGTTCCTCCCGATCTCAAC	ACCTTGACGTGGTGAAAGTC	55.0	50.0	58.0	58.1	1567-1625 bp	1.0	85.3
rrn16-trnV-GAC_p5	GTGTTCCTCCCGATCTCAAC	CCTTGACGTGGTGAAAGTCA	55.0	50.0	58.0	58.1	1566-1624 bp	1.0	85.3