Hotspot

trnR-ACG-rrn5

Genome · 57117-57221 · 105 bp

Back to Calocedrus

Pi 0.0571
MarkerSeek score 77.10
Primer available yes
Label trnR-ACG-rrn5

Feature Metadata

label_name
trnR-ACG-rrn5
feature_type
igs
region
Genome
length_bp
105
variable_sites
6
pi
0.0571
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnR-ACG-rrn5_p1 CCACGTGCTCTAATCCTCTG AATCCATCCCGAACTTGGTG 55.0 50.0 57.8 57.9 224-238 bp 1.0 79.3
trnR-ACG-rrn5_p2 CCACGTGCTCTAATCCTCTG ACCAATCCATCCCGAACTTG 55.0 50.0 57.8 57.9 227-241 bp 1.0 79.3
trnR-ACG-rrn5_p3 CCACGTGCTCTAATCCTCTG CAATCCATCCCGAACTTGGT 55.0 50.0 57.8 57.9 225-239 bp 1.0 79.3
trnR-ACG-rrn5_p4 CCACGTGCTCTAATCCTCTG CACCAATCCATCCCGAACTT 55.0 50.0 57.8 57.9 228-242 bp 1.0 79.3
trnR-ACG-rrn5_p5 AGTAGTGAACTGTTGTGGGC AATCCATCCCGAACTTGGTG 50.0 50.0 57.7 57.9 281-295 bp 1.0 78.6