Hotspot

psaA-ycf3

Genome · 38577-39076 · 500 bp

Back to Calocedrus

Pi 0.016

MarkerSeek score 66.10

Primer available yes

Label psaA-ycf3

Feature Metadata

label_name: psaA-ycf3
feature_type: igs
region: Genome
length_bp: 500
variable_sites: 8
pi: 0.016
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaA-ycf3_p1	AGAAATGACCGGGTTTAGCC	TGGTTTGATCAAGCTGCTGA	50.0	45.0	57.9	57.7	702-704 bp	1.0	77.8
psaA-ycf3_p2	TGGGTCCCTTAGCTAGTGTC	TGGTTTGATCAAGCTGCTGA	55.0	45.0	58.1	57.7	726-728 bp	1.0	77.7
psaA-ycf3_p3	GGGTCCCTTAGCTAGTGTCT	TGGTTTGATCAAGCTGCTGA	55.0	45.0	57.8	57.7	725-727 bp	1.0	77.5
psaA-ycf3_p4	TTGGGTCCCTTAGCTAGTGT	TGGTTTGATCAAGCTGCTGA	50.0	45.0	57.7	57.7	727-729 bp	1.0	76.1
psaA-ycf3_p5	AGAAATGACCGGGTTTAGCC	CTTGGTTTGATCAAGCTGCTG	50.0	47.6	57.9	58.1	704-706 bp	1.0	71.6