Hotspot

psbA-trnK-UUU

Genome · 1063-1558 · 496 bp

Back to Calocedrus

Pi 0.00855

MarkerSeek score 66.10

Primer available yes

Label psbA-trnK-UUU

Feature Metadata

label_name: psbA-trnK-UUU
feature_type: igs
region: Genome
length_bp: 496
variable_sites: 4
pi: 0.00855
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.94
markerseek_score: 66.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	GTGCTAGTGATCCAATCGCA	GAAAGCCGTGTGCAATGAAA	50.0	45.0	58.1	57.9	659-687 bp	1.0	82.3
psbA-trnK-UUU_p2	GTGCTAGTGATCCAATCGCA	CACGGTTTGGGGAGAGATTT	50.0	50.0	58.1	57.8	631-659 bp	1.0	81.9
psbA-trnK-UUU_p3	ATTCCATAGGCTTGCGCTTT	GAAAGCCGTGTGCAATGAAA	45.0	45.0	58.2	57.9	633-661 bp	1.0	81.0
psbA-trnK-UUU_p4	AGTGCTAGTGATCCAATCGC	GAAAGCCGTGTGCAATGAAA	50.0	45.0	57.8	57.9	660-688 bp	1.0	80.9
psbA-trnK-UUU_p5	AGTGCTAGTGATCCAATCGC	CACGGTTTGGGGAGAGATTT	50.0	50.0	57.8	57.8	632-660 bp	1.0	80.5