primer_id	label_name	rank	fwd_seq	rev_seq	fwd_len	rev_len	fwd_gc	rev_gc	fwd_tm	rev_tm	fwd_self_any_th	rev_self_any_th	primer3_penalty	target_start	target_end	amplicon_size	cross_species_success_rate	primer_score
psbA-trnK-UUU_p1	psbA-trnK-UUU	1	GTGCTAGTGATCCAATCGCA	GAAAGCCGTGTGCAATGAAA	20	20	50.0	45.0	58.1	57.9	3.6	0.0	0.192766	1063	1558	659-687	1.000000	82.3
psbA-trnK-UUU_p2	psbA-trnK-UUU	2	GTGCTAGTGATCCAATCGCA	CACGGTTTGGGGAGAGATTT	20	20	50.0	50.0	58.1	57.8	3.6	0.0	0.260398	1063	1558	631-659	1.000000	81.9
psbA-trnK-UUU_p3	psbA-trnK-UUU	3	ATTCCATAGGCTTGCGCTTT	GAAAGCCGTGTGCAATGAAA	20	20	45.0	45.0	58.2	57.9	9.7	0.0	0.364981	1063	1558	633-661	1.000000	81.0
psbA-trnK-UUU_p4	psbA-trnK-UUU	4	AGTGCTAGTGATCCAATCGC	GAAAGCCGTGTGCAATGAAA	20	20	50.0	45.0	57.8	57.9	0.0	0.0	0.363955	1063	1558	660-688	1.000000	80.9
psbA-trnK-UUU_p5	psbA-trnK-UUU	5	AGTGCTAGTGATCCAATCGC	CACGGTTTGGGGAGAGATTT	20	20	50.0	50.0	57.8	57.8	0.0	0.0	0.431587	1063	1558	632-660	1.000000	80.5
trnD-GUC-trnY-GUA_p1	trnD-GUC-trnY-GUA	1	TTGACAGGGCGGTACTCTAA	CCTACGCTGGTTCAAATCCA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.180463	28493	28705	282-282	1.000000	80.0
trnD-GUC-trnY-GUA_p2	trnD-GUC-trnY-GUA	2	TTGACAGGGCGGTACTCTAA	ATGCCTACGCTGGTTCAAAT	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.201283	28493	28705	285-285	1.000000	79.8
trnD-GUC-trnY-GUA_p3	trnD-GUC-trnY-GUA	3	AGGGCGGTACTCTAACCAAT	CCTACGCTGGTTCAAATCCA	20	20	50.0	50.0	58.1	57.9	0.0	0.0	0.232462	28493	28705	277-277	1.000000	79.6
trnD-GUC-trnY-GUA_p4	trnD-GUC-trnY-GUA	4	AGGGCGGTACTCTAACCAAT	ATGCCTACGCTGGTTCAAAT	20	20	50.0	45.0	58.1	57.9	0.0	0.0	0.253282	28493	28705	280-280	1.000000	79.4
trnD-GUC-trnY-GUA_p5	trnD-GUC-trnY-GUA	5	TTGACAGGGCGGTACTCTAA	GGGGACGGACTGTAAATTCG	20	20	50.0	55.0	58.1	58.4	0.0	0.0	0.424009	28493	28705	313-313	1.000000	77.9
psaA-ycf3_p1	psaA-ycf3	1	AGAAATGACCGGGTTTAGCC	TGGTTTGATCAAGCTGCTGA	20	20	50.0	45.0	57.9	57.7	6.3	19.9	0.399411	38577	39076	702-704	1.000000	77.8
psaA-ycf3_p2	psaA-ycf3	2	TGGGTCCCTTAGCTAGTGTC	TGGTTTGATCAAGCTGCTGA	20	20	55.0	45.0	58.1	57.7	10.0	19.9	0.413152	38577	39076	726-728	1.000000	77.7
psaA-ycf3_p3	psaA-ycf3	3	GGGTCCCTTAGCTAGTGTCT	TGGTTTGATCAAGCTGCTGA	20	20	55.0	45.0	57.8	57.7	0.0	19.9	0.437398	38577	39076	725-727	1.000000	77.5
psaA-ycf3_p4	psaA-ycf3	4	TTGGGTCCCTTAGCTAGTGT	TGGTTTGATCAAGCTGCTGA	20	20	50.0	45.0	57.7	57.7	10.0	19.9	0.612493	38577	39076	727-729	1.000000	76.1
psaA-ycf3_p5	psaA-ycf3	5	AGAAATGACCGGGTTTAGCC	CTTGGTTTGATCAAGCTGCTG	20	21	50.0	47.6	57.9	58.1	6.3	19.9	1.181568	38577	39076	704-706	1.000000	71.6
trnR-ACG-rrn5_p1	trnR-ACG-rrn5	1	CCACGTGCTCTAATCCTCTG	AATCCATCCCGAACTTGGTG	20	20	55.0	50.0	57.8	57.9	10.9	0.0	0.344678	57117	57221	224-238	1.000000	79.3
trnR-ACG-rrn5_p2	trnR-ACG-rrn5	2	CCACGTGCTCTAATCCTCTG	ACCAATCCATCCCGAACTTG	20	20	55.0	50.0	57.8	57.9	10.9	0.0	0.344678	57117	57221	227-241	1.000000	79.3
trnR-ACG-rrn5_p3	trnR-ACG-rrn5	3	CCACGTGCTCTAATCCTCTG	CAATCCATCCCGAACTTGGT	20	20	55.0	50.0	57.8	57.9	10.9	0.0	0.344678	57117	57221	225-239	1.000000	79.3
trnR-ACG-rrn5_p4	trnR-ACG-rrn5	4	CCACGTGCTCTAATCCTCTG	CACCAATCCATCCCGAACTT	20	20	55.0	50.0	57.8	57.9	10.9	0.0	0.344678	57117	57221	228-242	1.000000	79.3
trnR-ACG-rrn5_p5	trnR-ACG-rrn5	5	AGTAGTGAACTGTTGTGGGC	AATCCATCCCGAACTTGGTG	20	20	50.0	50.0	57.7	57.9	0.0	0.0	0.384888	57117	57221	281-295	1.000000	78.6
trnI-GAU-rrn16_p1	trnI-GAU-rrn16	1	GCGTTGATGAGCACATTGAA	CAAGGTAGCCGTACTGGAAG	20	20	45.0	55.0	57.4	57.7	0.0	7.8	0.912503	62707	63213	649-653	1.000000	76.4
trnI-GAU-rrn16_p2	trnI-GAU-rrn16	2	GCGTTGATGAGCACATTGAA	AGTGACTGGAGTGAAGTCGT	20	20	45.0	50.0	57.4	58.3	0.0	0.0	0.923623	62707	63213	671-675	1.000000	76.2
trnI-GAU-rrn16_p3	trnI-GAU-rrn16	3	GCGTTGATGAGCACATTGAA	AAGTCGTAACAAGGTAGCCG	20	20	45.0	50.0	57.4	57.6	0.0	0.0	0.976189	62707	63213	658-662	1.000000	75.8
trnI-GAU-rrn16_p4	trnI-GAU-rrn16	4	GCGTTGATGAGCACATTGAA	AACAAGGTAGCCGTACTGGA	20	20	45.0	50.0	57.4	58.4	0.0	7.8	0.981102	62707	63213	651-655	1.000000	75.8
trnI-GAU-rrn16_p5	trnI-GAU-rrn16	5	GCGTTGATGAGCACATTGAA	ACAAGGTAGCCGTACTGGAA	20	20	45.0	50.0	57.4	58.4	0.0	7.8	0.981102	62707	63213	650-654	1.000000	75.8
rrn16-trnV-GAC_p1	rrn16-trnV-GAC	1	GGGCAGGTTCTTACGCATTA	ACCTTGACGTGGTGAAAGTC	20	20	50.0	50.0	58.0	58.1	0.0	0.0	0.083215	64726	65556	997-1055	1.000000	85.9
rrn16-trnV-GAC_p2	rrn16-trnV-GAC	2	CCTACAGACACTTTACGCCC	ACCTTGACGTGGTGAAAGTC	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.056505	64726	65556	1433-1491	1.000000	85.5
rrn16-trnV-GAC_p3	rrn16-trnV-GAC	3	CCTACAGACACTTTACGCCC	CCTTGACGTGGTGAAAGTCA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.057866	64726	65556	1432-1490	1.000000	85.5
rrn16-trnV-GAC_p4	rrn16-trnV-GAC	4	GTGTTCCTCCCGATCTCAAC	ACCTTGACGTGGTGAAAGTC	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.062075	64726	65556	1567-1625	1.000000	85.3
rrn16-trnV-GAC_p5	rrn16-trnV-GAC	5	GTGTTCCTCCCGATCTCAAC	CCTTGACGTGGTGAAAGTCA	20	20	55.0	50.0	58.0	58.1	0.0	0.0	0.063436	64726	65556	1566-1624	1.000000	85.3
ycf2-rpl23_p1	ycf2-rpl23	1	TGATTGTTTATTGGCGGAAGG	TCTTTGTTTTATTCGATCCCGA	21	22	42.9	36.4	56.9	55.8	0.0	0.0	6.308354	77987	78623	792-833	1.000000	52.0
ycf2-rpl23_p2	ycf2-rpl23	2	TGATTGTTTATTGGCGGAAGGA	TCTTTGTTTTATTCGATCCCGA	22	22	40.9	36.4	58.3	55.8	0.0	0.0	6.475688	77987	78623	792-833	1.000000	52.0
ycf2-rpl23_p3	ycf2-rpl23	3	TGATTGTTTATTGGCGGAAGG	TGATCTTTGTTTTATTCGATCCCG	21	24	42.9	37.5	56.9	57.6	0.0	0.0	6.499210	77987	78623	795-836	1.000000	52.0
ycf2-rpl23_p4	ycf2-rpl23	4	GATTGTTTATTGGCGGAAGGA	TCTTTGTTTTATTCGATCCCGA	21	22	42.9	36.4	56.6	55.8	0.0	0.0	6.585000	77987	78623	791-832	1.000000	52.0
ycf2-rpl23_p5	ycf2-rpl23	5	TTGATTGTTTATTGGCGGAAGG	TCTTTGTTTTATTCGATCCCGA	22	22	40.9	36.4	57.6	55.8	0.0	0.0	6.615799	77987	78623	793-834	1.000000	52.0
rpl36-rps11_p1	rpl36-rps11	1	AAGTGCCGCCTAATTCGTAG	CTTGTAGCTCTACGTGGCAC	20	20	50.0	55.0	58.1	58.4	0.0	7.8	0.433920	85129	85287	257-259	1.000000	79.0
rpl36-rps11_p2	rpl36-rps11	2	AAGTGCCGCCTAATTCGTAG	TCTTGTAGCTCTACGTGGCA	20	20	50.0	50.0	58.1	58.5	0.0	0.0	0.522688	85129	85287	258-260	1.000000	78.3
rpl36-rps11_p3	rpl36-rps11	3	CGAAAAGTGCCGCCTAATTC	CTTGTAGCTCTACGTGGCAC	20	20	50.0	55.0	57.8	58.4	0.0	7.8	0.543913	85129	85287	261-263	1.000000	78.1
rpl36-rps11_p4	rpl36-rps11	4	GAAAAGTGCCGCCTAATTCG	CTTGTAGCTCTACGTGGCAC	20	20	50.0	55.0	57.8	58.4	0.0	7.8	0.543913	85129	85287	260-262	1.000000	78.1
rpl36-rps11_p5	rpl36-rps11	5	AATACGTGCTTCTGTTCGCA	CTTGTAGCTCTACGTGGCAC	20	20	45.0	55.0	58.2	58.4	0.0	7.8	0.579097	85129	85287	288-290	1.000000	77.6
petA-psbJ_p1	petA-psbJ	1	GCATCTGTCGTTTTAGCACAG	ACCGGATTAGGATCTTCCTTG	21	21	47.6	47.6	57.9	56.9	0.0	9.5	3.235609	96025	96490	528-574	1.000000	59.7
petA-psbJ_p2	petA-psbJ	2	GCATCTGTCGTTTTAGCACAG	CCGGATTAGGATCTTCCTTGT	21	21	47.6	47.6	57.9	56.9	0.0	0.0	3.235609	96025	96490	527-573	1.000000	59.7
petA-psbJ_p3	petA-psbJ	3	AGCATCTGTCGTTTTAGCACA	ACCGGATTAGGATCTTCCTTG	21	21	42.9	47.6	58.2	56.9	0.0	9.5	3.360067	96025	96490	529-575	1.000000	58.7
petA-psbJ_p4	petA-psbJ	4	AGCATCTGTCGTTTTAGCACA	CCGGATTAGGATCTTCCTTGT	21	21	42.9	47.6	58.2	56.9	0.0	0.0	3.360067	96025	96490	528-574	1.000000	58.7
petA-psbJ_p5	petA-psbJ	5	GCATCTGTCGTTTTAGCACA	CCGGATTAGGATCTTCCTTGT	20	21	45.0	47.6	56.7	56.9	0.0	0.0	3.377138	96025	96490	527-573	1.000000	58.6
psbJ-psbL_p1	psbJ-psbL	1	AGGGATTCTTCCGGTGGTAT	TAGCCTCTATTGGGGGTTGT	20	20	50.0	50.0	57.8	57.7	0.0	2.3	0.472289	96614	96792	214-276	1.000000	83.8
psbJ-psbL_p2	psbJ-psbL	2	AGGGATTCTTCCGGTGGTAT	ACTAGCCTCTATTGGGGGTT	20	20	50.0	50.0	57.8	57.4	0.0	2.3	0.780906	96614	96792	216-278	1.000000	81.3
psbJ-psbL_p3	psbJ-psbL	3	GAGGGATTCTTCCGGTGGTA	TAGCCTCTATTGGGGGTTGT	20	20	55.0	50.0	58.5	57.7	0.0	2.3	0.787544	96614	96792	215-277	1.000000	81.3
psbJ-psbL_p4	psbJ-psbL	4	AGGGATTCTTCCGGTGGTAT	AGCCTCTATTGGGGGTTGTT	20	20	50.0	50.0	57.8	58.6	0.0	0.0	0.809067	96614	96792	213-275	1.000000	81.1
psbJ-psbL_p5	psbJ-psbL	5	GAGGGATTCTTCCGGTGGTA	ACTAGCCTCTATTGGGGGTT	20	20	55.0	50.0	58.5	57.4	0.0	2.3	1.096161	96614	96792	217-279	1.000000	78.8
psaJ-rpl33_p1	psaJ-rpl33	1	TTTTTCCCAGATGCCCTGAC	ACATTTCCACTCTTGGCCAT	20	20	50.0	45.0	58.1	57.4	4.9	6.1	0.687082	98900	99205	309-362	1.000000	81.1
psaJ-rpl33_p2	psaJ-rpl33	2	ATATCTTTCCACAGCGCCTG	ACATTTCCACTCTTGGCCAT	20	20	50.0	45.0	58.0	57.4	2.3	6.1	0.651396	98900	99205	385-438	1.000000	80.9
psaJ-rpl33_p3	psaJ-rpl33	3	CATATCTTTCCACAGCGCCT	ACATTTCCACTCTTGGCCAT	20	20	50.0	45.0	58.0	57.4	2.3	6.1	0.651396	98900	99205	386-439	1.000000	80.9
psaJ-rpl33_p4	psaJ-rpl33	4	GATGCCCTGACTTTTCCCTT	ACATTTCCACTCTTGGCCAT	20	20	50.0	45.0	57.8	57.4	0.0	6.1	0.832665	98900	99205	300-353	1.000000	80.0
psaJ-rpl33_p5	psaJ-rpl33	5	ACATATCTTTCCACAGCGCC	ACATTTCCACTCTTGGCCAT	20	20	50.0	45.0	58.3	57.4	2.3	6.1	0.942019	98900	99205	387-440	1.000000	78.6
rps12-clpP_p1	rps12-clpP	1	GCATACTCCCTTCCGTTGAG	GCAGGTTGTTTTTCTAGCGC	20	20	55.0	50.0	58.1	58.3	0.0	0.0	0.372211	101347	102486	1254-1319	1.000000	83.2
rps12-clpP_p2	rps12-clpP	2	ATTGGCTGTCTCGCATTTCT	GCAGGTTGTTTTTCTAGCGC	20	20	45.0	50.0	57.9	58.3	0.0	0.0	0.433337	101347	102486	1196-1261	1.000000	82.8
rps12-clpP_p3	rps12-clpP	3	CATACTCCCTTCCGTTGAGG	GCAGGTTGTTTTTCTAGCGC	20	20	55.0	50.0	57.4	58.3	0.0	0.0	0.917582	101347	102486	1253-1318	1.000000	78.8
rps12-clpP_p4	rps12-clpP	4	TACACCCTAGCGCATACTCC	GCAGGTTGTTTTTCTAGCGC	20	20	55.0	50.0	58.7	58.3	11.5	0.0	0.988181	101347	102486	1265-1330	1.000000	78.3
rps12-clpP_p5	rps12-clpP	5	TGGCTGTCTCGCATTTCTAA	GCAGGTTGTTTTTCTAGCGC	20	20	45.0	50.0	57.2	58.3	0.0	0.0	1.074170	101347	102486	1194-1259	1.000000	77.7
ycf1_p1	ycf1	1	GCTATGGCTGGTTGCTTTAG	GACCTTTAAGTCGTTGGGAGA	20	21	50.0	47.6	56.9	57.3	0.0	0.0	2.816365	104754	104873	204-204	1.000000	62.6
ycf1_p2	ycf1	2	GCTATGGCTGGTTGCTTTAG	AGACCTTTAAGTCGTTGGGAG	20	21	50.0	47.6	56.9	57.3	0.0	0.0	2.823422	104754	104873	205-205	1.000000	62.5
ycf1_p3	ycf1	3	GCTATGGCTGGTTGCTTTAG	ACCTTTAAGTCGTTGGGAGA	20	20	50.0	45.0	56.9	56.1	0.0	0.0	3.048307	104754	104873	203-203	1.000000	60.7
ycf1_p4	ycf1	4	GCTATGGCTGGTTGCTTTAG	AGACCTTTAAGTCGTTGGGA	20	20	50.0	45.0	56.9	56.1	0.0	0.0	3.048307	104754	104873	205-205	1.000000	60.7
ycf1_p5	ycf1	5	GCTATGGCTGGTTGCTTTAG	GACCTTTAAGTCGTTGGGAGAA	20	22	50.0	45.5	56.9	58.0	0.0	0.0	3.144552	104754	104873	204-204	1.000000	59.9
trnL-CAA-accD_p1	trnL-CAA-accD	1	CACGCGACACTCAAAATGTC	TTCTCGTATGTGCTTCTGGC	20	20	50.0	50.0	58.1	58.0	15.9	0.0	0.072786	112403	113770	1681-1807	1.000000	89.7
trnL-CAA-accD_p2	trnL-CAA-accD	2	GACACGCGACACTCAAAATG	TTCTCGTATGTGCTTCTGGC	20	20	50.0	50.0	58.1	58.0	15.9	0.0	0.072786	112403	113770	1683-1809	1.000000	89.6
trnL-CAA-accD_p3	trnL-CAA-accD	3	CACGCGACACTCAAAATGTC	AGTATGGGATCGGTAGTGGG	20	20	50.0	55.0	58.1	58.0	15.9	0.0	0.073763	112403	113770	1754-1880	1.000000	89.4
trnL-CAA-accD_p4	trnL-CAA-accD	4	CACGCGACACTCAAAATGTC	GACGCTGGTGTCGAACTAAT	20	20	50.0	50.0	58.1	58.0	15.9	0.0	0.070471	112403	113770	1955-2081	1.000000	88.9
trnL-CAA-accD_p5	trnL-CAA-accD	5	GACACGCGACACTCAAAATG	GACGCTGGTGTCGAACTAAT	20	20	50.0	50.0	58.1	58.0	15.9	0.0	0.070471	112403	113770	1957-2083	1.000000	88.9
chlL-trnH-GUG_p1	chlL-trnH-GUG	1	CCGATTCCGCCTTTTCCATA	CCAAGGCAGTGGATTGTGAA	20	20	50.0	50.0	58.0	58.4	0.0	1.1	0.418552	126444	126589	231-236	1.000000	80.1
chlL-trnH-GUG_p2	chlL-trnH-GUG	2	CCGATTCCGCCTTTTCCATA	GTAGCCAAGTGGTTCCAAGG	20	20	50.0	55.0	58.0	58.5	0.0	0.0	0.503478	126444	126589	245-250	1.000000	79.2
chlL-trnH-GUG_p3	chlL-trnH-GUG	3	TTACCGATTCCGCCTTTTCC	CCAAGGCAGTGGATTGTGAA	20	20	50.0	50.0	58.3	58.4	0.0	1.1	0.638818	126444	126589	234-239	1.000000	78.3
chlL-trnH-GUG_p4	chlL-trnH-GUG	4	CCGCCTTTTCCATAAACTGC	CCAAGGCAGTGGATTGTGAA	20	20	50.0	50.0	57.7	58.4	0.0	1.1	0.658372	126444	126589	225-230	1.000000	78.2
chlL-trnH-GUG_p5	chlL-trnH-GUG	5	TTACCGATTCCGCCTTTTCC	GTAGCCAAGTGGTTCCAAGG	20	20	50.0	55.0	58.3	58.5	0.0	0.0	0.723743	126444	126589	248-253	1.000000	77.4