Hotspot

rps4

Genome · 75021-75644 · 624 bp

Back to Callitropsis

Pi 0.0112

MarkerSeek score 65.20

Primer available yes

Label rps4

Feature Metadata

label_name: rps4
feature_type: gene
region: Genome
length_bp: 624
variable_sites: 7
pi: 0.0112
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps4_p1	TCATTCCAATATTCGTGCTGT	ACAGAGGAACAGATGTGCAG	38.1	50.0	55.7	57.5	762 bp	1.0	50.3
rps4_p2	TCATTCCAATATTCGTGCTGTT	ACAGAGGAACAGATGTGCAG	36.4	50.0	56.4	57.5	762 bp	1.0	48.3
rps4_p3	TCATTCCAATATTCGTGCTGT	CAGAGGAACAGATGTGCAGA	38.1	50.0	55.7	57.2	761 bp	1.0	48.0
rps4_p4	ATCATTCCAATATTCGTGCTGT	ACAGAGGAACAGATGTGCAG	36.4	50.0	56.2	57.5	763 bp	1.0	46.6
rps4_p5	TCATTCCAATATTCGTGCTGTT	CAGAGGAACAGATGTGCAGA	36.4	50.0	56.4	57.2	761 bp	1.0	46.0