Hotspot

rpl16

Genome · 8856-10099 · 1244 bp

Back to Callitropsis

Pi 0.00884

MarkerSeek score 63.80

Primer available yes

Label rpl16

Feature Metadata

label_name: rpl16
feature_type: gene
region: Genome
length_bp: 1244
variable_sites: 11
pi: 0.00884
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 63.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl16_p1	AAACATCTTGGAGCAGTGCT	CCAATTGTTACCTCGGCAGA	45.0	50.0	57.7	57.9	1348-1353 bp	1.0	77.5
rpl16_p2	AAACATCTTGGAGCAGTGCT	CTCCAATTGTTACCTCGGCA	45.0	50.0	57.7	57.9	1350-1355 bp	1.0	77.5
rpl16_p3	AAACATCTTGGAGCAGTGCT	TCCAATTGTTACCTCGGCAG	45.0	50.0	57.7	57.9	1349-1354 bp	1.0	77.5
rpl16_p4	AAACATCTTGGAGCAGTGCT	CCTCCAATTGTTACCTCGGC	45.0	55.0	57.7	58.6	1351-1356 bp	1.0	73.4
rpl16_p5	AAACATCTTGGAGCAGTGCT	TCCTCCAATTGTTACCTCGG	45.0	50.0	57.7	56.9	1352-1357 bp	1.0	69.8