Hotspot

chlN-ndhJ

Genome · 120765-121237 · 473 bp

Back to Callitropsis

Pi 0.0148

MarkerSeek score 64.60

Primer available yes

Label chlN-ndhJ

Feature Metadata

label_name: chlN-ndhJ
feature_type: igs
region: Genome
length_bp: 473
variable_sites: 7
pi: 0.0148
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
chlN-ndhJ_p1	GACTTGGGTCGAACCACTTT	AGAAAGTTGGATTGGGTGGC	50.0	50.0	58.0	58.4	585-606 bp	1.0	77.7
chlN-ndhJ_p2	GACTTGGGTCGAACCACTTT	GCCAGAAAGTTGGATTGGGT	50.0	50.0	58.0	58.4	588-609 bp	1.0	77.7
chlN-ndhJ_p3	GACTTGGGTCGAACCACTTT	TGCCAGAAAGTTGGATTGGG	50.0	50.0	58.0	58.4	589-610 bp	1.0	77.7
chlN-ndhJ_p4	GCCAATGCAAGAGACGTACT	AGAAAGTTGGATTGGGTGGC	50.0	50.0	58.3	58.4	648-669 bp	1.0	75.7
chlN-ndhJ_p5	GCCAATGCAAGAGACGTACT	GCCAGAAAGTTGGATTGGGT	50.0	50.0	58.3	58.4	651-672 bp	1.0	75.7