Hotspot

trnV-GAC-rrn16

Genome · 52247-52706 · 460 bp

Back to Callitropsis

Pi 0.0239

MarkerSeek score 61.60

Primer available yes

Label trnV-GAC-rrn16

Feature Metadata

label_name: trnV-GAC-rrn16
feature_type: igs
region: Genome
length_bp: 460
variable_sites: 11
pi: 0.0239
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 61.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnV-GAC-rrn16_p1	ACCTTGACGTGGTGAAAGTC	GGGCAGGTTCTTACGCATTA	50.0	50.0	58.1	58.0	624-741 bp	1.0	80.6
trnV-GAC-rrn16_p2	CCTTGACGTGGTGAAAGTCA	GGGCAGGTTCTTACGCATTA	50.0	50.0	58.1	58.0	623-740 bp	1.0	80.6
trnV-GAC-rrn16_p3	ACCTTGACGTGGTGAAAGTC	TACGCATTACTCACCCGTTC	50.0	50.0	58.1	57.7	613-730 bp	1.0	78.6
trnV-GAC-rrn16_p4	CCTTGACGTGGTGAAAGTCA	TACGCATTACTCACCCGTTC	50.0	50.0	58.1	57.7	612-729 bp	1.0	78.6
trnV-GAC-rrn16_p5	ACCTTGACGTGGTGAAAGTC	CTTACGCATTACTCACCCGT	50.0	50.0	58.1	57.7	615-732 bp	1.0	78.6